GLYCOSIDASES IN ORGANIC-SOLVENTS .2. TRANSGALACTOSYLATION CATALYZED BY POLYETHYLENE GLYCOL-MODIFIED BETA-GALACTOSIDASE

被引：19

作者：

BEECHER, JE ^{[1
]}

ANDREWS, AT ^{[1
]}

VULFSON, EN ^{[1
]}

机构：

[1] INST FOOD RES, DEPT BIOTECHNOL & ENZYMOL, READING RG2 9AT, ENGLAND

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1990年 / 12卷 / 12期

关键词：

Galactosylation in organic solvents; PEG-modification; β-galactosidase;

D O I：

10.1016/0141-0229(90)90116-8

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

β-d-Galactoside galactohydrolase (E.C. 3.2.1.23) was chemically modified with 1,1′-carbonyldiimidazole-activated polyethylene glycols (MW 2,000, 8,000, and 20,000). The modified β-galactosidases had over 50% of amino groups coupled to polyethylene glycol but retained over 50% of the original activity. The hydrophobically modified enzymes were soluble in chlorinated organic solvents in which transferase activity has been demonstrated. The transferase activity, its dependency on water content, and the thermostability of all three modified enzymes were compared. © 1990.

引用

页码：955 / 959

页数：5

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