SKELETAL KERATAN SULFATE CHAIN MOLECULAR-WEIGHT CALIBRATION BY HIGH-PERFORMANCE GEL-PERMEATION CHROMATOGRAPHY

被引：16

作者：

DICKENSON, JM

MORRIS, HG

NIEDUSZYNSKI, IA

HUCKERBY, TN

机构：

[1] UNIV LANCASTER,INST ENVIRONM & BIOL SCI,DIV BIOL SCI,LANCASTER LA1 4YQ,ENGLAND

[2] UNIV LANCASTER,DEPT CHEM,LANCASTER LA1 4YA,ENGLAND

来源：

ANALYTICAL BIOCHEMISTRY | 1990年 / 190卷 / 02期

关键词：

D O I：

10.1016/0003-2697(90)90192-C

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method has been developed for the molecular sizing of skeletal keratan sulfate chains using an HPLC gelpermeation chromatography system. Keratan sulfate chains and keratanase-derived oligosaccharides were prepared from the nucleus pulposus of bovine intervertebral disc (6-year-old animals). A Bio-Gel TSK 30 XL column eluted in 0.2 m NaCl and at 30°C was calibrated with keratan sulfate oligosaccharides of known size as well as 3H-end-labeled keratan sulfate chains to yield the relationship log10Mr = 4.588 - 2.128Kav which is applicable in the range Kav = 0.15-0.65. © 1990.

引用

页码：271 / 275

页数：5

共 26 条

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