CONSTRUCTION AND PROPERTIES OF A FAMILY OF PACYC184-DERIVED CLONING VECTORS COMPATIBLE WITH PBR322 AND ITS DERIVATIVES

被引：452

作者：

BARTOLOME, B

JUBETE, Y

MARTINEZ, E

DELACRUZ, F

机构：

[1] Departamento de Biología Molecular, Universidad de Cantabria, Cardenal Herrera Oria s/n

来源：

GENE | 1991年 / 102卷 / 01期

关键词：

RECOMBINANT DNA; P15A ORI REPLICON; INTERMEDIATE COPY NUMBER; LACZP; SP6 AND T7 BACTERIOPHAGE PROMOTERS;

D O I：

10.1016/0378-1119(91)90541-I

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A family of cloning vectors derived from plasmid pACYC184 and, therefore, compatible with pBR322 and its derivatives (especially the pUC family of vectors), is described. They all contain a multiple cloning site (MCS) and the lacZ-alpha-reporter gene for easy cloning. They have been grouped in three sets: (i) six of the vectors contain a chloramphenicol-resistance (Cm(R))-encoding gene and each a different MCS with 16 unique restriction sites overall; (ii) another six vectors contain a kanamycin-resistance (KM(R))-encoding gene and the same six MCS; and (iii) two Cm(R) vectors that contain the SP6 and T7 promoters flanking the MCS and lacZ-alpha-reporter gene of pUC18/19.

引用

页码：75 / 78

页数：4

共 23 条

[1] ANALYSIS OF THE VARIABLE ENDPOINTS GENERATED BY ONE-ENDED TRANSPOSITION OF TN21 [J].