STRUCTURE OF TRANSCRIPTION ELONGATION COMPLEXES INVIVO

被引：88

作者：

KAINZ, M

ROBERTS, J

机构：

[1] Section of Biochemistry, Molecular, and Cell Biology, Biotechnology Building, Cornell University, Ithaca

来源：

SCIENCE | 1992年 / 255卷 / 5046期

关键词：

D O I：

10.1126/science.1536008

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The opening of duplex DNA in the elongation phase of transcription by Escherichia coli RNA polymerase in vivo was detected at a regulatory site where a prolonged pause in transcription occurs. Single-stranded DNA in the transcription bubble was identified by its reactivity with potassium permanganate (KMnO4). The elongation structure in vivo was similar to that of transcription complexes made in vitro with some differences. The observed reactivity to KMnO4 of the DNA template strand was consistent with the existence of an RNA-DNA hybrid of about 12 nucleotides.

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页码：838 / 841

页数：4

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