EXPRESSION OF RECOMBINANT HUMAN GLUTATHIONE-REDUCTASE IN EUKARYOTIC CELLS AFTER DNA-MEDIATED GENE-TRANSFER

被引：6

作者：

CHOLIN, S

TONOKI, H

HANSEN, TN

LEDLEY, FD

机构：

[1] BAYLOR COLL MED,DEPT CELL BIOL,1 BAYLOR PLAZA,HOUSTON,TX 77030

[2] BAYLOR COLL MED,DEPT PEDIAT,HOUSTON,TX 77030

[3] BAYLOR COLL MED,HOWARD HUGHES MED INST,HOUSTON,TX 77030

来源：

BIOCHEMICAL MEDICINE AND METABOLIC BIOLOGY | 1993年 / 49卷 / 01期

关键词：

D O I：

10.1006/bmmb.1993.1012

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Glutathione reductase (EC 1.6.4.2) protects tissues from oxidant stress by catalyzing the NADPH-mediated reduction of glutathione disulfide to glutathione. We describe construction of a vector for DNA-mediated gene transfer and successful transient overexpression of human glutathione reductase cDNA in CHO cells. No expression was observed when the same vector was transiently transfected into NIH3T3 or LA4 cells or stably integrated in CHO cells. These results demonstrate the ability to constitute recombinant glutathione reductase expression in eukaryotic cells hut suggest that this expression may be toxic. © 1993 Academic Press. All rights reserved.

引用

页码：108 / 113

页数：6

共 19 条

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