A RAPID, AUTOMATED MICROTITER ASSAY FOR MEASURING NEUTRALIZATION OF HIV-1

被引：18

作者：

LAAL, S

BURDA, S

SHARPE, S

ZOLLAPAZNER, S

机构：

[1] VET AFFAIRS MED CTR,423 E 23RD ST,NEW YORK,NY 10010

[2] NYU MED CTR,NEW YORK,NY 10016

来源：

AIDS RESEARCH AND HUMAN RETROVIRUSES | 1993年 / 9卷 / 08期

关键词：

D O I：

10.1089/aid.1993.9.781

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A sensitive, rapid, and quantative ELISA for p24 is described, which can be used as the read-out to test for HIV-1 neutralization in the syncytium-forming microassay and can replace the counting of syncytia under the microscope. This assay can be used reliably for divergent strains of HIV-1, including those that do not induce syncytia. The new read-out permits evaluation of neutralization at 3 days rather than 5 days. Using this assay, the neutralizing activity of several new and previously described human monoclonal antibodies against HIV-1 was characterized.

引用

页码：781 / 785

页数：5

共 11 条

[1] GENERATION OF HUMAN MONOCLONAL-ANTIBODIES TO HUMAN IMMUNODEFICIENCY VIRUS
GORNY, MK
GIANAKAKOS, V
SHARPE, S
ZOLLAPAZNER, S
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (05) : 1624 - 1628
[2] INFECTION OF HTLV-III/LAV IN HTLV-I-CARRYING CELLS MT-2 AND MT-4 AND APPLICATION IN A PLAQUE-ASSAY
HARADA, S
KOYANAGI, Y
YAMAMOTO, N
[J]. SCIENCE, 1985, 229 (4713) : 563 - 566
[3] PRODUCTION OF HUMAN MONOCLONAL-ANTIBODIES SPECIFIC FOR CONFORMATIONAL AND LINEAR NON-V3 EPITOPES OF GP120
KARWOWSKA, S
GORNY, MK
BUCHBINDER, A
GIANAKAKOS, V
WILLIAMS, C
FUERST, T
ZOLLAPAZNER, S
[J]. AIDS RESEARCH AND HUMAN RETROVIRUSES, 1992, 8 (06) : 1099 - 1106
[4] DETECTION OF HIV-1 NEUTRALIZING ANTIBODIES BY A SIMPLE, RAPID, COLORIMETRIC ASSAY
LANGLOIS, AJ
MATTHEWS, TJ
WEINHOLD, KJ
CHAFFEE, S
HERSHFIELD, M
BOLOGNESI, DP
[J]. AIDS RESEARCH AND HUMAN RETROVIRUSES, 1988, 4 (01) : 63 - 69
[5] INDUCTION OF CD4-DEPENDENT CELL-FUSION BY THE HTLV-III/LAV ENVELOPE GLYCOPROTEIN
LIFSON, JD
FEINBERG, MB
REYES, GR
RABIN, L
BANAPOUR, B
CHAKRABARTI, S
MOSS, B
WONGSTAAL, F
STEIMER, KS
ENGLEMAN, EG
[J]. NATURE, 1986, 323 (6090) : 725 - 728
[6] RESTRICTED NEUTRALIZATION OF DIVERGENT HUMAN T-LYMPHOTROPIC VIRUS TYPE-III ISOLATES BY ANTIBODIES TO THE MAJOR ENVELOPE GLYCOPROTEIN
MATTHEWS, TJ
LANGLOIS, AJ
ROBEY, WG
CHANG, NT
GALLO, RC
FISCHINGER, PJ
BOLOGNESI, DP
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (24) : 9709 - 9713
[7] EVALUATION OF ANTIVIRAL DRUGS AND NEUTRALIZING ANTIBODIES TO HUMAN IMMUNODEFICIENCY VIRUS BY A RAPID AND SENSITIVE MICROTITER INFECTION ASSAY
MONTEFIORI, DC
ROBINSON, WE
SCHUFFMAN, SS
MITCHELL, WM
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1988, 26 (02) : 231 - 235
[8] SIMPLE, RAPID, QUANTITATIVE, SYNCYTIUM-FORMING MICROASSAY FOR THE DETECTION OF HUMAN IMMUNODEFICIENCY VIRUS NEUTRALIZING ANTIBODY
NARA, PL
HATCH, WC
DUNLOP, NM
ROBEY, WG
ARTHUR, LO
GONDA, MA
FISCHINGER, PJ
[J]. AIDS RESEARCH AND HUMAN RETROVIRUSES, 1987, 3 (03) : 283 - 302
[9] PREVALENCE, CLINICAL-SIGNIFICANCE, AND STRAIN SPECIFICITY OF NEUTRALIZING ANTIBODY TO THE HUMAN-IMMUNODEFICIENCY-VIRUS
PRINCE, AM
PASCUAL, D
KOSOLAPOV, LB
KUROKAWA, D
BAKER, L
RUBINSTEIN, P
[J]. JOURNAL OF INFECTIOUS DISEASES, 1987, 156 (02) : 268 - 272
[10] A HUMAN MONOCLONAL-ANTIBODY AGAINST THE CD4-BINDING SITE OF HIV-1 GP120 EXHIBITS POTENT, BROADLY NEUTRALIZING ACTIVITY
TILLEY, SA
HONNEN, WJ
RACHO, ME
HILGARTNER, M
PINTER, A
[J]. RESEARCH IN VIROLOGY, 1991, 142 (04): : 247 - 259

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