POINT MUTATION IN THE PRIBNOW BOX, THE MOLECULAR-BASIS OF BETA-LACTAMASE OVERPRODUCTION IN KLEBSIELLA-OXYTOCA

被引：45

作者：

FOURNIER, B

LU, CY

LAGRANGE, PH

KRISHNAMOORTHY, R

PHILIPPON, A

机构：

[1] UNIV PARIS 07,HOP ST LOUIS,MICROBIOL LAB,F-75475 PARIS 10,FRANCE

[2] HOP ROBERT DEBRE,INSERM,U120,F-75019 PARIS,FRANCE

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 1995年 / 39卷 / 06期

关键词：

D O I：

10.1128/AAC.39.6.1365

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Klebsiella oxytoca mutants resistant to a variety of beta-lactams were obtained in vitro on aztreonam. Constitutive p-lactamase production was much higher in the mutants than in the susceptible strains (75-fold). The only difference observed in these mutants compared with the susceptible strains were point mutations in the Pribnow box: a transversion (G-->T) in the first base for one mutant or a transition (G-->A) in the fifth base of the -10 consensus sequence for the other three mutants. The transcriptional output of the beta-lactamase gene (bla(OXY)) from the mutants was significantly higher than that of the bla(OXY) gene from the susceptible strains.

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页码：1365 / 1368

页数：4

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