DETERMINATION OF TISSUE INHIBITOR OF METALLOPROTEINASES-2 (TIMP-2) IN EXPERIMENTAL-ANIMALS USING MONOCLONAL-ANTIBODIES AGAINST TIMP-2-SPECIFIC OLIGOPEPTIDES

被引：12

作者：

FUJIMOTO, N

TOKAI, H

IWATA, K

OKADA, Y

HAYAKAWA, T

机构：

[1] FUJI CHEM IND CO LTD,RES LABS 1,TAKAOKA,TOYAMA 933,JAPAN

[2] KANAZAWA UNIV,CANC RES INST,DEPT MOLEC IMMUNOL & PATHOL,KANAZAWA,ISHIKAWA 920,JAPAN

[3] AICHI GAKUIN UNIV,SCH DENT,DEPT BIOCHEM,CHIKUSA KU,NAGOYA,AICHI 464,JAPAN

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1995年 / 187卷 / 01期

关键词：

TISSUE INHIBITOR OF METALLOPROTEINASES-2; MATRIX METALLOPROTEINASE; ENZYME IMMUNOASSAY; MONOCLONAL ANTIBODY;

D O I：

10.1016/0022-1759(95)00164-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a one-step sandwich enzyme immunoassay (EIA) using monoclonal antibodies against oligopeptides of the human tissue inhibitor of metalloproteinases-2 (TIMP-2) (Fujimoto et al. (1993) Clin. Chim. Acta 220, 31). The present studies further demonstrated that the antibodies cross-react with TIMP-2 species of experimental animals including mouse, rat, guinea pig and rabbit. The detection of the TIMP-2 species in our ETA system was verified using rat TIMP-2 and the EIA was subsequently used to measure the animal TIMP-2 in the sera. Using human TIMP-2 as a standard, TIMP-2 levels in the sera of mouse, rat, guinea pig and rabbit were approximately 80, 200, 270 and 25 ng/ml, respectively.

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页码：33 / 39

页数：7

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