GALACTOSYLTRANSFERASE ACTIVITY IS NOT LOCALIZED TO THE BRUSH-BORDER MEMBRANE OF HUMAN SMALL-INTESTINE

被引：7

作者：

BOYLE, F ^{[1
]}

SNAPE, S ^{[1
]}

DUANE, P ^{[1
]}

COOK, N ^{[1
]}

PETERS, T ^{[1
]}

机构：

[1] MRC, CLIN RES CTR, DIV CLIN CELL BIOL, WATFORD RD, HARROW HA1 3UJ, MIDDX, ENGLAND

来源：

BIOSCIENCE REPORTS | 1986年 / 6卷 / 02期

关键词：

D O I：

10.1007/BF01115003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A recent report [Roth et al. (1985) J. Cell Biol. 100: 118-125], using immunocytochemical techniques, calimed that human duodenal galactosyltransferase is located predominantly on the external aspect of enterocyte brush border membranes. Analytical subcellular fractionation by sucrose density gradient centrifugation of human jejunum biopsy homogenates demonstrated that galactosyltransferase activity is localized to the Golgi fraction (equilibrium density of 1.14 g cm-3) and is not found in significant amounts in the brush border membrane (equilibrium density of 1.22 g cm-3).

引用

页码：171 / 175

页数：5

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