ANALYSIS OF DNA-STRUCTURE BY HYDROXYAPATITE COLUMNS AND ETHIDIUM-BROMIDE FLUORESCENCE TECHNIQUES - COMPARATIVE-STUDY AND EFFECT ON DNA BINDING

被引：18

作者：

DAVIS, P

BURRINGTON, M

RUSSELL, AS

MORGAN, AR

机构：

[1] University of Alberta, Canadian Arthritis and Rheumatism Society

[2] Rheumatic Disease Unit, University of Alberta

[3] Department of Medicine, University of Alberta, Edmonton, Alberta

来源：

ARTHRITIS AND RHEUMATISM | 1978年 / 21卷 / 04期

关键词：

D O I：

10.1002/art.1780210402

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Seven duplex DNA preparations have been structurally analyzed by hydroxyapatite column chromatography and an ethidium bromide fluorescence technique. Significant contamination of one preparation with single‐stranded DNA was detected by hydroxyapatite column chromatography. Five of the other six preparations were found to contain significant single‐stranded regions by the ethidium bromide fluorescence technique. Synthetic poly dAT was found to be duplex in structure. The presence of single‐stranded regions considerably influenced DNA binding results in a radioimmunoassay. Copyright © 1978 American College of Rheumatology

引用

页码：407 / 413

页数：7

共 27 条

[1]

AARDEN LA, 1975, SCAND J RHEUMATOL, P12

[2] IMMUNOLOGY OF DNA .2. EFFECT OF SIZE AND STRUCTURE OF ANTIGEN ON FARR ASSAY [J].