IMMUNOCYTOCHEMICAL LOCALIZATION OF ACTIN IN EPITHELIAL-CELLS OF RAT SMALL-INTESTINE BY LIGHT AND ELECTRON-MICROSCOPY

被引:27
作者
HAGEN, SJ [1 ]
TRIER, JS [1 ]
机构
[1] HARVARD UNIV, CTR DIGEST DIS, BOSTON, MA 02115 USA
关键词
D O I
10.1177/36.7.3290330
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
We used post-embedding immunocytochemical techniques and affinity-purified anti-actin antibody to evaluate localization of actin epithelial cells of small intestine by fluorescence and electron microscopy. Small intestine was fixed with 2% formaldehyde-0.1% glutaraldehyde and embedded in Lowicryl K4M. One-.mu.m or thin sections were stained with antibody followed by rhodamine- or colloidal gold-labeled goat anti-rabbit IgG, respectively. Label was present overlying microvilli, the apical terminal web, and the cytoplasm directly adjacent to occluding and intermediate junctions. Label was associated with outer mitochondrial membranes of all cells and the supranuclear Golgi region of goblet cells. Lateral cytoplasmic interdigitations between mature cells and subplasmalemmal filaments next to intrusive cells were densely labeled. The cytoplasm adjacent to unplicated domains of lateral membrane was focally labeled. Label was prominent over organized filaments bundles within the subplasmalemmal web at the base of mature cells, whereas there was focal labeling of the cytoplasm adjacent to the basal membrane of undifferentiated cells. Basolateral epithelial cell processes were labeled. Label was focally present overlying the cellular ground substance. Our results demonstrate that actin is distributed in a distinctive fashion within intestinal epithelial cells. This distribution suggests that in addition to its function as a structural protein, actin may participate in regulation of epithelial tight junction permeabilty, in motile processes including migration of cells from the crypt to the villus tip, in accommodation of intrusive intraepithelial cells and in adhesion of cells to one another and to their substratum.
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页码:717 / 727
页数:11
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