CRYSTALLIZATION OF CYTOCHROME C-553 IN AEROBICALLY GROWN PSEUDOMONAS DENITRIFICANS

被引：20

作者：

IWASAKI, H

SHIDARA, S

机构：

[1] Biological Institute, Faculty of Science, Nagoya University, Nagoya

来源：

JOURNAL OF BIOCHEMISTRY | 1969年 / 66卷 / 06期

关键词：

D O I：

10.1093/oxfordjournals.jbchem.a129207

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cytochrome c-553 was crystallized from the soluble fraction of aerobically grown cells of Pseudomonas denitrificans. Its molecular weight was estimated to be 45,000. It had two haem prosthetic groups per molecule; one of them showed reduced α-peak at about 551 mμ and the other at about 555 mμ. Their E0' (pH7) values were estimated to be -0.09 V and between 0 and -0.09 V, respectively. The cytochrome was readily autooxidizable. The ferro-form reacted with CO, and the ferro-and ferri-forms reacted with KCN at pH 7. © 1969 BY THE JOURNAL OF BIOCHEMISTRY.

引用

页码：775 / &

共 17 条

[1] ESTIMATION OF MOLECULAR WEIGHTS OF PROTEINS BY SEPHADEX GEL-FILTRATION [J].