METHANOL AS A CONVENIENT INDUCER OF HEAT-SHOCK ELEMENT-DIRECTED HETEROLOGOUS GENE-EXPRESSION IN YEAST

被引：3

作者：

KIRK, N

PIPER, PW

机构：

[1] UNIV LONDON UNIV COLL,DEPT BIOCHEM & MOLEC BIOL,LONDON WC1E 6BT,ENGLAND

[2] UNIV LONDON UNIV COLL,DEPT CHEM & BIOCHEM ENGN,LONDON WC1E 6BT,ENGLAND

来源：

BIOTECHNOLOGY LETTERS | 1991年 / 13卷 / 07期

关键词：

SACCHAROMYCES-CEREVISIAE; ALKANOLS; ETHANOL;

D O I：

10.1007/BF01049201

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The addition of sublethal concentrations of methanol to yeast cultures was found to give strong induction of a lacZ gene under heat shock element (HSE) control. Methanol addition is therefore an easily-executed alternative to temperature upshift for the induction of HSE-directed gene expression, although in this study it gave slightly lower levels of induction compared to heat shock.

引用

页码：465 / 470

页数：6

共 12 条

[1] TRANSCRIPTIONAL REGULATION OF SSA3, AN HSP70 GENE FROM SACCHAROMYCES-CEREVISIAE [J].