EFFECTS OF BETA-CYCLODEXTRIN IN THE MOBILE PHASE ON THE RETENTION AND INDIRECT DETECTION OF NONELECTROLYTES IN REVERSED-PHASE LIQUID-CHROMATOGRAPHY .2. STEROIDS

被引:22
作者
AGNUS, B [1 ]
SEBILLE, B [1 ]
GOSSELET, M [1 ]
机构
[1] UNIV PARIS 12,PHYSICOCHIM BIOPOLYMERES LAB,CNRS,UM 27,F-94320 THIAIS,FRANCE
来源
JOURNAL OF CHROMATOGRAPHY | 1991年 / 552卷 / 1-2期
关键词
D O I
10.1016/S0021-9673(01)95974-6
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The effect of beta-cyclodextrin on the chromatographic retention time of two steroids, pregnanolone and progesterone is described. The apparent formation constant of the inclusion complexes has been calculated, and the optimization of indirect photodetection of pregnanolone, in reversed-phase chromatography, has been investigated. The presence of beta-cyclodextrin in the mobile phase appears essential to enhance the detection of pregnanolone.
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页码:583 / 592
页数:10
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