TEMPORAL DIFFERENCES IN DESMIN EXPRESSION BETWEEN MYOBLASTS FROM EMBRYONIC AND ADULT CHICKEN SKELETAL-MUSCLE

被引:38
作者
YABLONKAREUVENI, Z
NAMEROFF, M
机构
[1] Department of Biological Structure, School of Medicine, University of Washington, Seattle, Washington
关键词
D O I
10.1111/j.1432-0436.1990.tb00452.x
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Desmin expression by myoblasts cultured from embryonic and adult chicken breast muscle was examined employing indirect immunofluorescence. The study was performed in conjunction with [3H]thymidine auto-radiography and analysis of skeletal myosin expression in order to determine whether the desmin-expressing cells were terminally differentiated. Following 2 h of labeling with [3H]thymidine, 0.55%, 2.60%, and 15.10% of the cells in mass cultures from 10-day-old embryos, 18-day-old embryos and adults, respectively, incorporated [3H]thymidine and were desmin-positive but did not express skeletal-muscle-specific myosin. Using the same approach we determined that 0.07%, 1.25%, and 7.59% of the mononucleated cells in myogenic clones from 10-day-old embryos, 18-day-old embryos and adults, respectively, were desmin-positive, myosin-negative, [3H]thymidine-positive. We suggest that these desmin-positive, myosin-negative myoblasts are proliferating cells, and we conclude that the progeny of adult myoblasts exhibit more desmin-expressing cells of this type than embryonic myoblasts do. © 1990, International Society of Differentiation. All rights reserved.
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页码:21 / 28
页数:8
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