INDUCTION OF REINITIATION OF MEIOSIS IN AMPHIBIAN BUFO AND XENOPUS OOCYTES BY INJECTION OF M-PHASE EXTRACTS OF CILIATE TETRAHYMENA NEEDS THE RECIPIENT PROTEIN-SYNTHESIS

被引：6

作者：

FUJISHIMA, M

KODAMA, I

HORI, M

KATSU, Y

IMAI, R

机构：

[1] Biological Institute, Faculty of Science, Yamaguchi University, Yamaguchi

来源：

EXPERIMENTAL CELL RESEARCH | 1991年 / 193卷 / 01期

关键词：

D O I：

10.1016/0014-4827(91)90550-E

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We show here that germinal vesicle breakdown of amphibian Bufo and Xenopus oocytes can be induced if ciliate Tetrahymena extracts are injected into them. The activity of meiosis-reinitiation-inducing factor (MRIF) appeared only at M-phase of a synchronously dividing culture, indicating that this MRIF has an important function for induction of M-phase in the mitotic cell cycle. MRIF of Tetrahymena differed from MPF (M-phase-promoting factor), because its action on the induction of GVBD was inhibited by cycloheximide and it could not induce GVBD in starfish oocytes by microinjection. MPF activity was not detected in extracts of vegetatively growing Tetrahymena. Preliminary experiments showed that MRIF was a heat-labile, Ca2+-sensitive, and trypsin-sensitive soluble protein. © 1991.

引用

页码：155 / 160

页数：6

共 21 条

[1] CDC2 IS A COMPONENT OF THE M-PHASE SPECIFIC HISTONE-H1 KINASE - EVIDENCE FOR IDENTITY WITH MPF [J].