MODE OF GROWTH OF RHIZOPUS-OLIGOSPORUS ON A MODEL SUBSTRATE IN SOLID-STATE FERMENTATION

被引:23
作者
MITCHELL, DA
GREENFIELD, PF
DOELLE, HW
机构
[1] Department of Chemical Engineering, University of Queensland
[2] Department of Microbiology, University of Queensland
关键词
D O I
10.1007/BF01200942
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The growth of Rhizopus oligosporus on a model solid substrate consisting of cassava starch and other nutrients embedded in a kappa-carrageenan matrix was followed microscoplcally. There were two distinct growth phases: (1) germination of spores and development of mycellum to form a loose network over the whole substrate surface; (2) increase in the density of the mycellum. Although some penetrative hyphae were produced, the biomass was malnly restricted to the surface. Consequently, starch utilization was greatest near the surface. Based on these observations a descriptive model for the growth of Rhizopus ollgosporus on the model substrate was proposed. The five steps are: (1) release of glucoamylase by the mycelium; (2) diffusion of the glucoamylase to the starch; (3) hydrolysis of the starch by the glucoamylase, releasing glucose; (4) diffusion of glucose; (5) absorption of glucose by the mycellum at the surface to produce new blomass. © 1990 Rapid Communications of Oxford Ltd.
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页码:201 / 208
页数:8
相关论文
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