FERTILIZABILITY OF CRYOPRESERVED ZONA-FREE HAMSTER OVA

被引:27
作者
FLEMING, AD
YANAGIMACHI, R
YANAGIMACHI, H
机构
[1] Department of Anatomy and Reproductive Biology, University of Hawaii Medical School, Honolulu
来源
GAMETE RESEARCH | 1979年 / 2卷 / 04期
关键词
cryopreservation; fertilization; ova; spermatozoa;
D O I
10.1002/mrd.1120020408
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Mature unfertilized ova from superovulated hamsters were freed from all investments and frozen at −50°C. They were cooled at about 1°C/min to 0°C then at 0.8° to 0.6°C/min to −50°C. At 0°C, dimethyl sulfoxide was added to a final concentration of 1.25 M. The ova were stored at −50°C for up to four months. Thawing was performed at 2–4°C/min and followed by several washes with insemination medium. Approximately 90% of the ova were normal in appearance after thawing. The frozen and thawed ova with normal appearance could be penetrated by hamster or human spermatozoa at a rate comparable to unfrozen controls. The ability of hamster ova to tolerate storage at a relatively convenient temperature (−50°C) for long periods (tested for up to four months) makes possible their shipment at low cost to institutions lacking this resource. There they can be used for basic biological studies of sperm–egg interaction or in the clinical assessment of human sperm quality. Copyright © 1979 Alan R. Liss, Inc.
引用
收藏
页码:357 / 366
页数:10
相关论文
共 20 条
[1]   FERTILIZING-CAPACITY OF HUMAN SPERMATOZOA EVALUATED BY ACTUAL PENETRATION OF FOREIGN EGGS [J].
BARROS, C ;
GONZALEZ, J ;
HERRERA, E ;
BUSTOSOBREGON, E .
CONTRACEPTION, 1978, 17 (01) :87-92
[2]  
BARROS C, 1979, ANDROLOGIA, V11, P197
[3]  
BARROS C, 1974, PHYSL GENETICS REP B, P3
[4]  
BIGGERS J. D., 1971, Methods in mammalian embryology, P86
[5]   FERTILIZABILITY OF RABBIT OVA AND THE EFFECTS OF TEMPERATURE INVITRO ON THEIR SUBSEQUENT FERTILIZATION AND ACTIVATION INVIVO [J].
CHANG, MC .
JOURNAL OF EXPERIMENTAL ZOOLOGY, 1952, 121 (02) :351-381
[6]   PLAQUE FORMATION AND ISOLATION OF PURE LINES WITH POLIOMYELITIS VIRUSES [J].
DULBECCO, R ;
VOGT, M .
JOURNAL OF EXPERIMENTAL MEDICINE, 1954, 99 (02) :167-182
[7]   PENETRATION OF ZONA-FREE EGGS BY SPERMATOZOA OF DIFFERENT SPECIES [J].
HANADA, A ;
CHANG, MC .
BIOLOGY OF REPRODUCTION, 1972, 6 (02) :300-+
[8]  
HANADA A, 1976, J REPROD FERTIL, V46, P239, DOI 10.1530/jrf.0.0460239
[9]  
IMAI H, 1977, J REPROD FERTIL, V51, P495, DOI 10.1530/jrf.0.0510495
[10]  
KANWAR KC, 1979, FERTIL STERIL, V31, P321