ANALYSIS OF GENTAMICIN SULFATE IN PHARMACEUTICAL SPECIALITIES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：49

作者：

FREEMAN, M

HAWKINS, PA

LORAN, JS

STEAD, JA

机构：

[1] Roussel Laboratories Limited, Covingham, Swindon, Wiltshire, Kingfisher Drive

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1979年 / 2卷 / 09期

关键词：

D O I：

10.1080/01483917908060139

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This paper describes an H.P.L.C. method for the assay of gentamicin sulphate, utilising pre-column derivatisation with an o-phthalaldehyde/thioglycolIic acid reagent, ion-paired chromatography of the derivative on a Hypersil O.D.S. column and U.V. detection. The four major components of gentamicin are resolved (Gentamicins C1, Cla/ C2 and C2a) and evidence is presented to show that the method is stability indicating. The application of this technique to formulated products is described and figures for precision and accuracy as well as stability results are given. Copyright © 1979 by Marcel Dekker, Inc.

引用

页码：1305 / 1317

页数：13

共 16 条

[1] CHEMICAL DERIVATIZATION FOR THE LIQUID-CHROMATOGRAPHY OF COMPOUNDS OF PHARMACEUTICAL INTEREST [J].