DEVELOPING BRONCHOPULMONARY EPITHELIUM OF THE HUMAN FETUS SECRETES FLUID

被引:40
作者
MCCRAY, PB
BETTENCOURT, JD
BASTACKY, J
机构
[1] CHILDRENS HOSP,DEPT PULM,OAKLAND,CA 94609
[2] CHILDRENS HOSP,PULM RES INST,OAKLAND,CA 94609
[3] UNIV CALIF BERKELEY,LAWRENCE BERKELEY LAB,BERKELEY,CA 94720
来源
AMERICAN JOURNAL OF PHYSIOLOGY | 1992年 / 262卷 / 03期
关键词
FETAL PULMONARY EPITHELIAL CELLS; SECRETION; ION TRANSPORT; HUMAN FETAL LUNG DEVELOPMENT; FETAL CELLS; ORGAN CULTURE;
D O I
10.1152/ajplung.1992.262.3.L270
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
We studied human fetal lung tissue in submersion organ culture to determine whether the bronchopulmonary epithelium secretes fluid during development. In this system the acinar tubules continued to grow, secrete fluid, and become progressively dilated. Baseline transepithelial potential differences (PSI(t)) of -0.5 to -11 mV (mean, -3.8 mV, lumen negative, n = 27) were measured with microelectrodes after 3-8 days in culture, suggesting active electrolyte transport. Bumetanide (500-mu-M), an inhibitor of chloride secretion in other systems, decreased the basal PSI(t) from -5 +/- 1.5 to -3.2 +/- 1.6 (SE) mV (P < 0.05, n = 6), suggesting that chloride transport contributed to the voltage. Isoproterenol (5-mu-M) increased the baseline PSI(t) from -5.6 +/- 2.1 to -9.2 +/- 2.5 (SE) mV (P < 0.05, n = 4). Subsequent addition of bumetanide inhibited the isoproterenol-induced stimulation of the PSI(t) by 20% (P < 0.05). 8-(4-chlorophenylthio)adenosine 3',5'-cyclic monophosphate. (CPT-cAMP, 50-mu-M) and 3-isobutyl 1-methylxanthine (IBMX, 100-mu-M) had similar effects, causing an increase in the PSI(t) from -2.2 +/- 0.5 to -8 +/- 1.6 (SE) mV, an effect that was inhibited by the addition of bumetanide (P < 0.005, n = 6). Both isoproterenol and CPT-cAMP/IBMX produced significant increases in the percentage luminal area of the explants at 12 and 24 h after exposure compared with control. We conclude that 1) the developing bronchopulmonary epithelium (acinar tubules) contributes to lung fluid production in the human fetus, 2) fetal lung fluid secretion is chloride dependent, and 3) chloride secretion and fluid secretion may be stimulated by a beta-agonist and cAMP.
引用
收藏
页码:L270 / L279
页数:10
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