HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF 5-HYDROXYINDOLES BY POSTCOLUMN FLUORESCENCE DERIVATIZATION

被引：42

作者：

ISHIDA, J ^{[1
]}

IIZUKA, R ^{[1
]}

YAMAGUCHI, M ^{[1
]}

机构：

[1] FUKUOKA UNIV,FAC PHARMACEUT SCI,NANAKUMA,JOHNAN KU,FUKUOKA 81401,JAPAN

来源：

ANALYST | 1993年 / 118卷 / 02期

关键词：

5-HYDROXYINDOLE AND 5-HYDROXYINDOL-3-YLACETIC ACID; POSTCOLUMN FLUORESCENCE REACTION; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY; BENZYLAMINE; HUMAN URINE;

D O I：

10.1039/an9931800165

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A selective and sensitive high-performance liquid chromatographic method with post-column fluorescence detection has been developed for the determination of 5-hydroxyindoles. Five biogenic 5-hydroxyindoles (5-hydroxytryptophan, 5-hydroxytryptamine, 5-hydroxyindol-3-ylacetic acid, 5-hydroxytryptophol and N-acetyl-5-hydroxytryptamine) were separated by isocratic elution on a reversed-phase column, TSKgel ODS-80Tm, and then converted into fluorescent derivatives by post-column reaction with benzylamine and potassium hexacyanoferrate(III) reagents. The detection limits (signal-to-noise ratio = 3) for the indoles were in the range 140-470 fmol per injection volume (100 mul). The method was applied to the determination of 5-hydroxyindol-3-ylacetic acid in human urine, with direct sample injection.

引用

页码：165 / 169

页数：5

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