PROTEIN SECONDARY STRUCTURE DETERMINATION BY NMR - APPLICATION WITH RECOMBINANT HUMAN CYCLOPHILIN

被引：65

作者：

WUTHRICH, K ^{[1
]}

SPITZFADEN, C ^{[1
]}

MEMMERT, K ^{[1
]}

WIDMER, H ^{[1
]}

WIDER, G ^{[1
]}

机构：

[1] SANDOZ PHARMA AG, PRAKLIN FORSCH, CH-4002 BASEL, SWITZERLAND

来源：

FEBS LETTERS | 1991年 / 285卷 / 02期

关键词：

PROTEIN STRUCTURE; SECONDARY STRUCTURE; NUCLEAR MAGNETIC RESONANCE; NMR STRUCTURE; CYCLOPHILIN;

D O I：

10.1016/0014-5793(91)80808-G

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

It is a unique trait of the NMR method for protein structure determination that a description of the polypeptide secondary structure can be obtained at an early stage and quite independently of the complete structure calculation. In this paper the procedures used for secondary structure determination are reviewed and placed in perspective relative to the other steps in a complete three-dimensional structure determination. As an illustration the identification of the regular secondary structure elements in human cyclophilin is described.

引用

页码：237 / 247

页数：11

共 57 条

[1] INTERACTIVE PROGRAM FOR INVESTIGATION OF PROTEIN STRUCTURES BASED ON H-1-NMR EXPERIMENTS [J].

BILLETER, M ;

ENGELI, M ;

WUTHRICH, K .

JOURNAL OF MOLECULAR GRAPHICS, 1985, 3 (03) :79-&

[2] SEQUENTIAL RESONANCE ASSIGNMENTS IN PROTEIN H-1 NUCLEAR MAGNETIC-RESONANCE SPECTRA - COMPUTATION OF STERICALLY ALLOWED PROTON PROTON DISTANCES AND STATISTICAL-ANALYSIS OF PROTON PROTON DISTANCES IN SINGLE-CRYSTAL PROTEIN CONFORMATIONS [J].

BILLETER, M ;

BRAUN, W ;

WUTHRICH, K .

JOURNAL OF MOLECULAR BIOLOGY, 1982, 155 (03) :321-346

[3] DISTANCE GEOMETRY AND RELATED METHODS FOR PROTEIN-STRUCTURE DETERMINATION FROM NMR DATA [J].

BRAUN, W .

QUARTERLY REVIEWS OF BIOPHYSICS, 1987, 19 (3-4) :115-157

[4] 4-DIMENSIONAL C-13/C-13-EDITED NUCLEAR OVERHAUSER ENHANCEMENT SPECTROSCOPY OF A PROTEIN IN SOLUTION - APPLICATION TO INTERLEUKIN 1-BETA [J].

CLORE, GM ;

KAY, LE ;

BAX, A ;

GRONENBORN, AM .

BIOCHEMISTRY, 1991, 30 (01) :12-18

[5] TWO-DIMENSIONAL H-1-NMR STUDY OF HUMAN UBIQUITIN - A MAIN CHAIN DIRECTED ASSIGNMENT AND STRUCTURE-ANALYSIS [J].

DISTEFANO, DL ;

WAND, AJ .

BIOCHEMISTRY, 1987, 26 (23) :7272-7281

[6] DETERMINATION OF THE SECONDARY STRUCTURE AND MOLECULAR TOPOLOGY OF INTERLEUKIN-1-BETA BY USE OF 2-DIMENSIONAL AND 3-DIMENSIONAL HETERONUCLEAR N-15-H-1 NMR-SPECTROSCOPY [J].

DRISCOLL, PC ;

GRONENBORN, AM ;

WINGFIELD, PT ;

CLORE, GM .

BIOCHEMISTRY, 1990, 29 (19) :4668-4682

[7] INDIVIDUAL ASSIGNMENTS OF AMIDE PROTON RESONANCES IN THE PROTON NMR-SPECTRUM OF THE BASIC PANCREATIC TRYPSIN-INHIBITOR [J].

DUBS, A ;

WAGNER, G ;

WUTHRICH, K .

BIOCHIMICA ET BIOPHYSICA ACTA, 1979, 577 (01) :177-194

[8]

ECCLES C, 1991, IN PRESS J BIOMOL NM

[9] MAIN-CHAIN-DIRECTED STRATEGY FOR THE ASSIGNMENT OF H-1-NMR SPECTRA OF PROTEINS [J].

ENGLANDER, SW ;

WAND, AJ .

BIOCHEMISTRY, 1987, 26 (19) :5953-5958

[10] HYDROGEN-EXCHANGE AND STRUCTURAL DYNAMICS OF PROTEINS AND NUCLEIC-ACIDS [J].

ENGLANDER, SW ;

KALLENBACH, NR .

QUARTERLY REVIEWS OF BIOPHYSICS, 1983, 16 (04) :521-655

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