RANDOM SEQUENCE OLIGONUCLEOTIDE PRIMERS DETECT POLYMORPHIC DNA PRODUCTS WHICH SEGREGATE IN INBRED STRAINS OF MICE

被引：70

作者：

WOODWARD, SR ^{[1
]}

SUDWEEKS, J ^{[1
]}

TEUSCHER, C ^{[1
]}

机构：

[1] BRIGHAM YOUNG UNIV,DEPT MICROBIOL,PROVO,UT 84602

来源：

MAMMALIAN GENOME | 1992年 / 3卷 / 02期

关键词：

D O I：

10.1007/BF00431249

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The random amplification of polymorphic DNA (RAPD) using primers of arbitrary nucleotide sequence has been extremely valuable in identifying heritable markers in a variety of systems. The present studies examined whether the RAPD technique can identify large numbers of polymorphisms that can be used to construct genetic maps in inbred strains of mice. By screening the inbred mouse strains C57BL/6J and DBA/2J with 481 random 10-mer oligonucleotide primers, we identified 95 polymorphisms and mapped 76 of these by use of the BXD series of recombinant inbred (RI) strains. The results clearly demonstrate that the RAPD technique allows for the identification of large numbers of DNA-based polymorphisms that distinguish these two inbred strains of mice, and that such markers can readily be used to construct molecular genetic linkage maps.

引用

页码：73 / 78

页数：6

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