ENDOPOLYGALACTURONASE IN APPLES (MALUS-DOMESTICA) AND ITS EXPRESSION DURING FRUIT RIPENING

被引:66
作者
WU, QD
SZAKACSDOBOZI, M
HEMMAT, M
HRAZDINA, G
机构
[1] CORNELL UNIV,INST FOOD SCI,GENEVA,NY 14456
[2] CORNELL UNIV,DEPT HORT,GENEVA,NY 14456
关键词
D O I
10.1104/pp.102.1.219
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The activity of polygalacturonase (PG) has been detected in ripe McIntosh apples (Malus domestica Borkh. cv McIntosh) both by enzyme activity measurement and immunoblotting using an anti-tomato-PG antibody preparation. PG activity increased during fruit ripening and remained steady, or decreased slightly, after 5 months of controlled atmospheric storage. The enzyme had a relative molecular weight of 45,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and 56,000 to 61,000 when determined by gel filtration. Viscosity and reducing end group measurements with a commercial pectin preparation showed that the enzyme is endo acting. In RNA and DNA blot hybridization experiments, a full-length tomato PG cDNA hybridized with the apple RNA and DNA, showing the identity of genes encoding the activity of the enzyme in tomato and apple.
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页码:219 / 225
页数:7
相关论文
共 37 条
[1]  
Abeles F. B., 1985, Ethylene and plant development, P1
[2]   CELLULASE ACTIVITY IN DEVELOPING APPLE FRUITS [J].
ABELES, FB ;
BILES, CL .
SCIENTIA HORTICULTURAE, 1991, 47 (1-2) :77-87
[3]   PURIFICATION AND CHARACTERIZATION OF THE POLYGALACTURONASES OF TOMATO FRUITS [J].
ALI, ZM ;
BRADY, CJ .
AUSTRALIAN JOURNAL OF PLANT PHYSIOLOGY, 1982, 9 (02) :155-169
[4]   COPPER ENZYMES IN ISOLATED CHLOROPLASTS - POLYPHENOLOXIDASE IN BETA-VULGARIS [J].
ARNON, DI .
PLANT PHYSIOLOGY, 1949, 24 (01) :1-15
[5]  
BARTLEY IM, 1978, PHYTOCHEMISTRY, V12, P213
[6]   ULTRASTRUCTURAL CHANGES IN THE CELL-WALLS OF RIPENING APPLE AND PEAR FRUIT [J].
BENARIE, R ;
KISLEV, N ;
FRENKEL, C .
PLANT PHYSIOLOGY, 1979, 64 (02) :197-202
[7]   THE TOMATO POLYGALACTURONASE GENE AND RIPENING-SPECIFIC EXPRESSION IN TRANSGENIC PLANTS [J].
BIRD, CR ;
SMITH, CJS ;
RAY, JA ;
MOUREAU, P ;
BEVAN, MW ;
BIRD, AS ;
HUGHES, S ;
MORRIS, PC ;
GRIERSON, D ;
SCHUCH, W .
PLANT MOLECULAR BIOLOGY, 1988, 11 (05) :651-662
[8]  
BOURNE M. C., 1965, Food Technology, V19, P113
[9]  
BOURNE MC, 1969, FOOD TECHNOL-CHICAGO, V23, P59
[10]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3