NARK IS A NITRITE-EXTRUSION SYSTEM INVOLVED IN ANAEROBIC NITRATE RESPIRATION BY ESCHERICHIA-COLI

被引:78
作者
ROWE, JJ
UBBINKKOK, T
MOLENAAR, D
KONINGS, WN
DRIESSEN, AJM
机构
[1] UNIV GRONINGEN, DEPT MICROBIOL, 9751 NN HAREN, NETHERLANDS
[2] UNIV DAYTON, DEPT BIOL, DAYTON, OH 45469 USA
关键词
D O I
10.1111/j.1365-2958.1994.tb01044.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Escherichia coli can use nitrate as a terminal electron acceptor for anaerobic respiration. A polytopic membrane protein, termed NarK, has been implicated in nitrate uptake and nitrite excretion and is thought to function as a nitrate/nitrite antiporter. The longest-lived radioactive isotope of nitrogen, N-13-nitrate (half-life = 9.96 min) and the nitrite-sensitive fluorophore N-(ethoxycarbonylmethyl)-6-methoxyquinolinium bromide have now been used to define the function of NarK. At low concentrations of nitrate, NarK mediates the electrogenic excretion of nitrite rather than nitrate/nitrite exchange. This process prevents intracellular accumulation of toxic levels of nitrite and allows further detoxification in the periplasm through the action of nitrite reductase.
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收藏
页码:579 / 586
页数:8
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