COLORIMETRIC MICROWELL PLATE HYBRIDIZATION ASSAY FOR DETECTION OF AMPLIFIED MYCOBACTERIUM-TUBERCULOSIS DNA FROM SPUTUM SAMPLES

被引：15

作者：

CHO, SN

VANDERVLIET, GME

PARK, S

BAIK, SH

KIM, SK

CHONG, Y

KOLK, AHJ

KLATSER, PR

KIM, JD

机构：

[1] YONSEI UNIV,COLL MED,DEPT MED,SEOUL 120752,SOUTH KOREA

[2] YONSEI UNIV,COLL MED,DEPT CLIN PATHOL,SEOUL 120752,SOUTH KOREA

[3] ROYAL TROP INST,DEPT BIOMED RES,1105 AZ AMSTERDAM,NETHERLANDS

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1995年 / 33卷 / 03期

关键词：

D O I：

10.1128/JCM.33.3.752-754.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We developed a colorimetric microwell plate hybridization assay (CoMPHA) for the specific detection of 5'-biotinylated amplified Mycobacterium tuberculosis DNA. The optical densities of the CoMPHA corresponded to the initial amounts of purified template DNA. Here, we show that the CoMPHA is useful in distinguishing the PCR-positive and PCR-negative samples.

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页码：752 / 754

页数：3

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