METABOLISM OF 4-BENZOTHIENYL N-METHYLCARBAMATE (MOBAM) IN RATS - BALANCE STUDY AND URINARY METABOLITE SEPARATION

The excretion patterns, tissue levels, and ionexchange chromatography of the urinary metabolites of Mobam have been determined in rats. Rats (two groups, 14 per group) were dosed with Mobam-14C (ring-labeled) at 2.0 and 13.0 mg. per kg. of body weight. After 24 hours, 87% (2.0 mg. per kg.) and 76% (13.0 mg. per kg.) of the 14C dose were excreted as water-soluble compounds in the urine. Carcasses of rats from both groups sacrificed at 3 days contained less than 1.0% of the radioactive dose. Tissue residues were fairly evenly distributed, decreasing in the 13.0 mg. per kg. group from an average of 0.5 p.p.m. (wet tissue) after 2 days to 0.2 p.p.m. after 8 days. Anion exchange chromatography on AE cellulose separated the radioactivity in the urine into four fractions. Two metabolites in the urine, 4-benzothienyl sulfate and 4-benzothienyl glucuronide, accounted for 83 and 87% of the excreted 14C from rats dosed at 2.0 and 13.0 mg. per kg., respectively. These metabolites were isolated and purified by liquid ion-exchange chromatography, paper chromatography, gel filtration, and gas-liquid chromatography. © 1969, American Chemical Society. All rights reserved.