BRAIN ADENOSINE PRODUCTION IN THE RAT DURING 60 SECONDS OF ISCHEMIA

In rats, cerebral perfusion pressure was altered abruptly by aortic transection to determine the production by ischemic brain of adenosine and its metabolites, inosine and hypoxanthine. Brain samples were obtained after 0, 5, 10, 15, 30, and 60 seconds of ischemia. Also measured were ATP, ADP, AMP, phosphocreatine (PCr), lactate, and pyruvate. Blood pressure was monitored continuously, and arterial PO2, PCO2, and pH were measured just prior to induction of ischemia. Adenosine was elevated to 2.30 ± 0.31 (SE) nmol/g at 5 seconds from a control value of 0.96 ± 0.07. A significant elevation of adenosine continued to 60 seconds (5.50 ± 1.24). Furthermore, inosine showed a progressive upward trend during the entire 60 seconds of ischemia, whereas no change in hypoxanthine occurred between the moment of transection (31.81 ± 2.01 nmol/g) and 60 seconds of ischemia (34.72 ± 2.93). PCr decreased by 1.24 μmol/g within the first 5 seconds. After the onset of hypotension, significant changes did not occur in AMP and ADP until 30 seconds, and in ATP and pyruvate until 60 seconds after aortic transection; lactate was elevated by 10 seconds. The rapid rise of cerebral adenosine within 5 seconds after the onset of ischemia supports a role for adenosine in the regulation of cerebral blood flow.