INHIBITION OF GLUTAMATE RELEASE BY ARACHIDONIC-ACID IN RAT CEREBROCORTICAL SYNAPTOSOMES

被引：27

作者：

HERRERO, I ^{[1
]}

MIRASPORTUGAL, MT ^{[1
]}

SANCHEZPRIETO, J ^{[1
]}

机构：

[1] UNIV COMPLUTENSE MADRID, FAC VET, DEPT BIOQUIM, E-28040 MADRID, SPAIN

来源：

JOURNAL OF NEUROCHEMISTRY | 1991年 / 57卷 / 02期

关键词：

ARACHIDONIC ACID; GLUTAMATE RELEASE; 4-AMINOPYRIDINE; TETRAETHYLAMMONIUM; CYTOSOLIC FREE CA-2+; PLASMA MEMBRANE POTENTIAL;

D O I：

10.1111/j.1471-4159.1991.tb03805.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The action of arachidonic acid on glutamate release in rat cerebrocortical synaptosomes was investigated. The Ca2+-dependent release of glutamate evoked by 4-aminopyridine (4-AP) was inhibited by arachidonic acid (0.5-10-mu-M), but the KCl-evoked release was not modified. The Ca2+-independent release of glutamate was insensitive to low concentrations of arachidonic acid, but higher concentrations of this free fatty acid (30-mu-M) induced a slow efflux of cytoplasmic glutamate. The decrease in the Ca2+-dependent release of glutamate by arachidonic acid was consistent with a reduction in both the depolarization and the subsequent rise in the cytoplasmic free Ca2+ concentration induced by 4-AP in the nerve terminal. The inhibitory action by arachidonic acid observed in glutamate release was reversed in the presence of the K+-channel blocker tetraethylammonium.

引用

页码：718 / 721

页数：4

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