CLONING AND NUCLEOTIDE-SEQUENCE OF A NEGATIVE REGULATOR GENE FOR KLEBSIELLA-AEROGENES ARYLSULFATASE SYNTHESIS AND IDENTIFICATION OF THE GENE AS FOLA

被引:22
作者
AZAKAMI, H [1 ]
SUGINO, H [1 ]
MUROOKA, Y [1 ]
机构
[1] HIROSHIMA UNIV,FAC ENGN,DEPT FERMENTAT TECHNOL,KAGAMIYAMA 1,HIGASHIHIROSHIMA 724,JAPAN
关键词
D O I
10.1128/JB.174.7.2344-2351.1992
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A negative regulator gene for synthesis of arylsulfatase in Klebsiella aerogenes was cloned. Deletion analysis showed that the regulator gene was located within a 1.6-kb cloned segment. Transfer of the plasmid, which contains the cloned fragment, into constitutive atsR mutant strains of K. aerogenes resulted in complementation of atsR; the synthesis of arylsulfatase was repressed in the presence of inorganic sulfate or cysteine, and this repression was relieved, in each case, by the addition of tyramine. The nucleotide sequence of the 1.6-kb fragment was determined. From the amino acid sequence deduced from the DNA sequence, we found two open reading frames. One of them lacked the N-terminal region but was highly homologous to the gene which codes for diadenosine tetraphosphatase (apaH) in Escherichia coli. The other open reading frame was located counterclockwise to the apaH-like gene. This gene was highly homologous to the gene which codes for dihydrofolate reductase (folA) in E. coli. We detected 30 times more activity of dihydrofolate reductase in the K. aerogenes strains carrying the plasmid, which contains the arylsulfatase regulator gene, than in the strains without plasmid. Further deletion analysis showed that the K. aerogenes folA gene is consistent with the essential region required for the repression of arylsulfatase synthesis. Transfer of a plasmid containing the E. coli folA gene into atsR mutant cells of K. aerogenes resulted in repression of the arylsulfatase synthesis. Thus, we conclude that the folA gene codes a negative regulator for the ats operon.
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页码:2344 / 2351
页数:8
相关论文
共 30 条
[1]   DEREPRESSION OF ARYLSULFATASE SYNTHESIS IN AEROBACTER-AEROGENES BY TYRAMINE [J].
ADACHI, T ;
MUROOKA, Y ;
HARADA, T .
JOURNAL OF BACTERIOLOGY, 1973, 116 (01) :19-24
[2]   CATABOLITE REPRESSION AND DEREPRESSION OF ARYLSULFATASE SYNTHESIS IN KLEBSIELLA-AEROGENES [J].
ADACHI, T ;
OKAMURA, H ;
MUROOKA, Y ;
HARADA, T .
JOURNAL OF BACTERIOLOGY, 1974, 120 (02) :880-885
[3]   REGULATION OF ARYLSULFATASE SYNTHESIS BY SULFUR-COMPOUNDS IN KLEBSIELLA-AEROGENES [J].
ADACHI, T ;
MUROOKA, Y ;
HARADA, T .
JOURNAL OF BACTERIOLOGY, 1975, 121 (01) :29-35
[4]   THE GENE FOR ESCHERICHIA-COLI DIADENOSINE TETRAPHOSPHATASE IS LOCATED IMMEDIATELY CLOCKWISE TO FOLA AND FORMS AN OPERON WITH KSGA [J].
BLANCHINROLAND, S ;
BLANQUET, S ;
SCHMITTER, JM ;
FAYAT, G .
MOLECULAR & GENERAL GENETICS, 1986, 205 (03) :515-522
[5]   CONSTRUCTION AND CHARACTERIZATION OF NEW CLONING VEHICLES .2. MULTIPURPOSE CLONING SYSTEM [J].
BOLIVAR, F ;
RODRIGUEZ, RL ;
GREENE, PJ ;
BETLACH, MC ;
HEYNEKER, HL ;
BOYER, HW ;
CROSA, JH ;
FALKOW, S .
GENE, 1977, 2 (02) :95-113
[6]  
DAVIS RW, 1980, MANUAL GENETIC ENG A
[7]   SULFATE ESTER FORMATION AND HYDROLYSIS - POTENTIALLY IMPORTANT YET OFTEN IGNORED ASPECT OF SULFUR CYCLE OF AEROBIC SOILS [J].
FITZGERALD, JW .
BACTERIOLOGICAL REVIEWS, 1976, 40 (03) :698-721
[8]  
HAMMALVAREZ SF, 1990, J BIOL CHEM, V265, P9850
[9]   STUDIES ON TRANSFORMATION OF ESCHERICHIA-COLI WITH PLASMIDS [J].
HANAHAN, D .
JOURNAL OF MOLECULAR BIOLOGY, 1983, 166 (04) :557-580
[10]   REPRESSION + INDUCTION OF ARYLSULPHATASE SYNTHESIS IN AEROBACTER AEROGENES [J].
HARADA, T ;
SPENCER, B .
BIOCHEMICAL JOURNAL, 1964, 93 (02) :373-&