THE POSITIONS OF 12 SIMPLE SEQUENCE REPEAT MARKERS RELATIVE TO REFERENCE LOCI ON MOUSE CHROMOSOME-16

被引：7

作者：

IRVING, NG ^{[1
]}

CITRON, MP ^{[1
]}

REEVES, RH ^{[1
]}

机构：

[1] JOHNS HOPKINS UNIV,SCH MED,DEPT PHYSIOL PHYSIOL 202,DEV GENET LAB,725 N WOLFE ST,BALTIMORE,MD 21205

来源：

MAMMALIAN GENOME | 1993年 / 4卷 / 07期

关键词：

D O I：

10.1007/BF00360586

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The genetic map positions of 12 simple sequence repeat (SSR) markers spanning mouse Chromosome (Chr) 16 were determined relative to reference markers on that chromosome. Interval mapping data were obtained with a panel of DNAs from two intersubspecific backcrosses. All but one of the markers were typed by use of nonradioactive polymerase chain reaction (PCR) products analyzed on agarose gels. The marker order was determined to be Prm-1, D16Mit9, Igl-1, D16Mit29, D16Mit1/D16Mit2, Smst, D16Mit4, D16Mit11, Gap43, D16Mit14, D16Mit30, D16Mit5, Pit-1, D16Mit27, D16H21S16 (formerly D2]S16h), D16Mit19, App, D16Mit7, Sod-1. Two of these markers mapped to the known human Chr 21 (HSA21)/Chr 16 conserved linkage group. Nine additional SSR markers could not be typed because they were not polymorphic (four markers), did not amplify MOLD/Rk DNA (three markers), or failed to give PCR products under a range of conditions (two markers). A subset of the most robust SSRs provide a useful marker set for the analysis of previously unmapped crosses.

引用

页码：364 / 367

页数：4

共 8 条

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