MINIMAL RESIDUAL DISEASE AFTER BONE-MARROW TRANSPLANT FOR CHRONIC MYELOID-LEUKEMIA DETECTED BY THE POLYMERASE CHAIN-REACTION

被引：49

作者：

CROSS, NCP ^{[1
]}

LIN, F ^{[1
]}

BUNGEY, J ^{[1
]}

GOLDMAN, JM ^{[1
]}

机构：

[1] HAMMERSMITH HOSP,ROYAL POSTGRAD MED SCH,LRF LEUKAEMIA UNIT,LONDON W12 ONN,ENGLAND

来源：

LEUKEMIA & LYMPHOMA | 1993年 / 11卷

关键词：

CML; BMT; MRD; PCR;

D O I：

10.3109/10428199309047861

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We describe the methodology and application of the polymerase chain reaction to detect BCR-ABL mRNA as a marker for CML cells. The technique is highly sensitive enabling the routine detection of 1 leukaemic cell in 10(5) or 10(6) normal cells and is therefore the most sensitive method available for detecting minimal residual disease. Analysis of marrow or blood from 80 patients after bone marrow transplantation for CML shows that residual leukaemia is often detectable for several months but that most subsequently become PCR negative. Patients who relapsed were all PCR positive before the detection of Philadelphia positive metaphases in bone marrow aspirates.

引用

页码：39 / 43

页数：5

共 7 条

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