THE IMMUNOGENIC PROPERTIES OF POLY(DI).POLY(DC) AND POLY(RI).POLY(DC) - ANALYSIS BY MONOCLONAL-ANTIBODIES

被引：14

作者：

BRAUN, RP ^{[1
]}

WOODSWORTH, ML ^{[1
]}

LEE, JS ^{[1
]}

机构：

[1] UNIV SASKATCHEWAN, DEPT BIOCHEM, SASKATOON S7N 0W0, SASKATCHEWAN, CANADA

来源：

MOLECULAR IMMUNOLOGY | 1986年 / 23卷 / 06期

关键词：

D O I：

10.1016/0161-5890(86)90107-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Eight monoclonal antibodies were prepared from a C57/black mouse which had been immunized with poly(rI) .cntdot. poly(dC). Two of the antibodies were specific for the RNA-DNA duplex but bound about 100-fold better to poly(rI) .cntdot. poly(dC) than to poly(rA) .cntdot. poly(dT). The other six antibodies were single-strand specific and bound to poly(rI) and in most cases to poly(dI) as well, but not to other single-stranded nucleic acids. Similarly, 10 monoclonal antibodies were produced from mice immunized with poly(dI) .cntdot. poly(dC). Nine of these were specific for poly(dI) while the other had a strong preference for poly(dC). Thus, in contrast to poly(rI) .cntdot. poly(dC), the all DNA duplex poly(dI) .cntdot. poly(dC) only elicits duplex specific antibodies at very low frequency, if at all.

引用

页码：685 / 691

页数：7

共 23 条

[1] MONOCLONAL MURINE ANTI-NUCLEIC ACID ANTIBODY WITH DOUBLE-STRANDED SPECIFICITY [J].

BALLARD, DW ;

VOSS, EW .

MOLECULAR IMMUNOLOGY, 1982, 19 (06) :793-799

[2] A MONOCLONAL-ANTIBODY TO THE DOUBLE-STRANDED POLYRIBONUCLEOTIDE COMPLEX POLY(A).POLY(U) [J].

DELAGE, G ;

NAHON, E ;

HUYNH, T ;

JEUSSET, J ;

LACOUR, F .

MOLECULAR IMMUNOLOGY, 1984, 21 (10) :939-944

[3] MONOCLONAL AUTOANTIBODIES - AN APPROACH TO STUDYING AUTOIMMUNE-DISEASE [J].

EILAT, D .

MOLECULAR IMMUNOLOGY, 1982, 19 (07) :943-955

[4] ANTIBODIES TO POLY(I).POLY(C) - PURIFICATION AND INTERACTION WITH POLYNUCLEOTIDES [J].

GUIGUES, M ;

LENG, M .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1976, 69 (02) :615-624

[5]

JACOB L, 1982, J IMMUNOL, V128, P895

[6] GENERATION AND CHARACTERIZATION OF 3 MURINE MONOCLONAL NUCLEOTIDE BINDING ANTI-SSDNA AUTOANTIBODIES [J].

KARDOST, RRP ;

BILLING, PA ;

VOSS, EW .

MOLECULAR IMMUNOLOGY, 1982, 19 (08) :963-972

[7]

KITAGAWA Y, 1982, MOL IMMUNOL, V19, P413

[8] MONOCLONAL-ANTIBODIES SHOWING SEQUENCE SPECIFICITY IN THEIR INTERACTION WITH SINGLE-STRANDED DNAS [J].

LEE, JS ;

LEWIS, JR ;

MORGAN, AR ;

MOSMANN, TR ;

SINGH, B .

NUCLEIC ACIDS RESEARCH, 1981, 9 (07) :1707-1721

[9] SPECIFICITY OF AUTOIMMUNE MONOCLONAL FAB FRAGMENTS BINDING TO SINGLE-STRANDED DEOXYRIBONUCLEIC-ACID [J].

LEE, JS ;

DOMBROSKI, DF ;

MOSMANN, TR .

BIOCHEMISTRY, 1982, 21 (20) :4940-4945

[10] A MONOCLONAL-ANTIBODY SPECIFIC FOR THE DUPLEX DNA POLY[D(TC)].POLY[D(GA)] [J].

LEE, JS ;

LATIMER, LJP ;

WOODSWORTH, ML .

FEBS LETTERS, 1985, 190 (01) :120-124

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