A DIRECT-READING DEVICE FOR MEASUREMENT OF PATCH-CLAMP MICROPIPETTE TIP DIAMETERS

被引：13

作者：

MARTIN, DK ^{[1
]}

COOK, DI ^{[1
]}

机构：

[1] UNIV SYDNEY,DEPT PHYSIOL,SYDNEY,NSW 2006,AUSTRALIA

来源：

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY | 1990年 / 417卷 / 03期

关键词：

Electronic device; Inner tip diameter; Micropipette; Patch clamp;

D O I：

10.1007/BF00370989

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

This paper describes an electronic device for the measurement of the diameter of the tip opening of micropipettes. The device measures the threshold pressure required to produce bubbles from the micropipette submerged in a liquid and displays the tip inner diameter in micrometres. We demonstrate that this technique can be used effectively in a range of liquids of different density, surface tension and viscosity and that the thickness of the glass from which the micropipettes are made does not influence the measurements. © 1990 Springer-Verlag.

引用

页码：255 / 258

页数：4

共 11 条

[1] NEW MICRO-ELECTRODE TECHNIQUES FOR INTRACELLULAR WORK IN SMALL CELLS [J].