IDENTIFICATION OF A NOVEL S-PHASE-SPECIFIC GENE DURING THE CELL-CYCLE IN SYNCHRONOUS CULTURES OF CATHARANTHUS-ROSEUS CELLS

被引:37
作者
ITO, M
KODAMA, H
KOMAMINE, A
机构
[1] Biological Institute, Faculty of Science, Tohoku University, Sendai
关键词
D O I
10.1111/j.1365-313X.1991.00141.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The cell-cycle specific cDNAs were isolated from a cDNA library prepared from cells in the S phase in the synchronous cultures of Catharanthus roseus. One of the isolated genes, which we refer to as cyc07, was analyzed in detail. The full-length cDNA of cyc07 contains an open reading frame of 735 nucleotides, encoding a protein of 245 amino acids with a molecular weight of 28356 Da. The protein predicted from the nucleotide sequence is highly basic, as are mammalian histones. cyc07 mRNA was detected specifically in cells at the S phase in synchronous cultures. The induction and accumulation of mRNA in the S phase were suppressed when DNA synthesis was inhibited by aphidicolin. In the intact plant, cyc07 mRNA was found preferentially in root tips that contained meristematic tissue. A databank search revealed that a sequence homologous to the nucleotide sequence of cyc07 cDNA is present in the downstream region of the SIR3 gene in the yeast genome. The amino acid sequence predicted from the corresponding region of the yeast genome exhibited significant homology with that of cyc07 protein. These similarities between cyc07 and the corresponding region in yeast suggest that the homologous sequence in yeast is a novel gene that is functionally homologous to cyc07. Our results presented here suggest the possibility that cyc07 may play a role in the proliferation of higher plant cells, in particular in the entry into or progression of the S phase of the cell cycle.
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页码:141 / 148
页数:8
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