ENDORPHIN-LIKE IMMUNOREACTIVITIES IN UNCULTURED AND CULTURED HUMAN PERIPHERAL-BLOOD MONONUCLEAR-CELLS

被引:18
作者
VANWOUDENBERG, AD [1 ]
HOL, EM [1 ]
WIEGANT, VM [1 ]
机构
[1] UNIV UTRECHT,DEPT PHARMACOL,RUDOLF MAGNUS INST,VONDELLAAN 6,3521 GD UTRECHT,NETHERLANDS
关键词
D O I
10.1016/0024-3205(92)90473-3
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
It has been reported that cells of the immune system produce and release considerable amounts of pro-opiomelanocortin (POMC) -derived peptides in response to coculture with a variety of stimulatory agents. The present study investigated whether extracts of human peripheral blood mononuclear cells (PBMC) contain immunoreactivity for beta-endorphin (beta-E) and related peptides. Using four endorphin RIA systems with different specificities, extracts of freshly isolated PBMC and PBMC cultured in the presence or absence of mitogens or of corticotropin releasing factor (CRF) and vasopressin (VP), were analyzed. With a radioimmunoassay (RIA) system directed to the midportion of beta-E, immunoreactivity (MPssE-IR) was readily detectable, although the concentration was extremely low (ca. 200 pg/10(7) cells). beta-E immunoreactivity (beta-E-IR) and alpha-endorphin immunoreactivity (alpha-E-IR), as determined in C-terminally directed RIA systems, were present in even lower concentrations. gamma-Endorphin immunoreactivity (gamma-E-IR) was hardly detectable. Of subsets enriched in T-cells, B-cells or monocytes, the highest concentration of MPss-IR was detected in extracts of monocytes. Coculture of PBMC with the mitogen Concanavalin A (Con A) or Phytohaemagglutinin (PHA) increased the amount of MPssE-IR in extracts of the cells. No increase in alpha-E-IR, however, was detected, whereas beta-E-IR was only increased in extracts of cells cultured in the presence of Con A. No increase, in any of the immunoreactivities, was observed in extracts of PBMC cultured with bacterial lipopolysaccharide (LPS) or with the combination of CRF and VP, both stimuli that have been reported to induce POMC peptides in cultured PBMC. The present data show that human PBMC contain endorphin-like immunoreactivity, but in very small amounts. The extremely low concentrations and the ineffectiveness of LPS and the combination of CRF and VP to increase the endorphin-like immunoreactivity raise questions about the reported capacity of PBMC to synthesize POMC-derived peptides.
引用
收藏
页码:705 / 714
页数:10
相关论文
共 24 条
[1]   DIFFERENTIAL-EFFECTS OF CISTERNA MAGNA CANNULATION ON BETA-ENDORPHIN LEVELS IN RAT PLASMA AND CEREBROSPINAL-FLUID [J].
BARNA, I ;
SWEEP, CGJ ;
VELDHUIS, HD ;
WIEGANT, VM .
ACTA ENDOCRINOLOGICA, 1988, 117 (04) :517-524
[2]   HUMAN-LEUKOCYTE INTERFERON - STRUCTURAL AND BIOLOGICAL RELATEDNESS TO ADRENOCORTICOTROPIC HORMONE AND ENDORPHINS [J].
BLALOCK, JE ;
SMITH, EM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (10) :5972-5974
[3]   NO EXPRESSION OF IR-ACTH ON STIMULATED PERIPHERAL-BLOOD LEUKOCYTES [J].
BOHME, MWJ ;
BECKER, K ;
SCHERBAUM, WA ;
JIRIKOWSKI, G ;
SPATHSCHWALBE, E ;
FEHM, HL ;
PFEIFFER, EF .
HORMONE AND METABOLIC RESEARCH, 1987, 19 (12) :670-671
[4]  
Burbach J, 1990, NEUROPEPTIDES BASICS, P45
[5]  
BURBACH JPH, 1984, FEBS LETT, V1666, P267
[6]   EXPRESSION OF PRO-OPIOMELANOCORTIN GENE AND QUANTIFICATION OF ADRENOCORTICOTROPIC HORMONE LIKE IMMUNOREACTIVITY IN HUMAN NORMAL PERIPHERAL MONONUCLEAR-CELLS AND LYMPHOID AND MYELOID MALIGNANCIES [J].
BUZZETTI, R ;
MCLOUGHLIN, L ;
LAVENDER, PM ;
CLARK, AJL ;
REES, LH .
JOURNAL OF CLINICAL INVESTIGATION, 1989, 83 (02) :733-737
[7]   PROOPIOMELANOCORTIN GENE IS EXPRESSED IN MANY NORMAL HUMAN-TISSUES AND IN TUMORS NOT ASSOCIATED WITH ECTOPIC ADRENOCORTICOTROPIN SYNDROME [J].
DEBOLD, CR ;
MENEFEE, JK ;
NICHOLSON, WE ;
ORTH, DN .
MOLECULAR ENDOCRINOLOGY, 1988, 2 (09) :862-870
[8]   VIRUS-INDUCED INCREASES IN PLASMA-CORTICOSTERONE [J].
DUNN, AJ ;
POWELL, ML ;
GASKIN, JM .
SCIENCE, 1987, 238 (4832) :1423-1424
[9]  
Eipper B A, 1980, Endocr Rev, V1, P1
[10]   ABSENCE OF FUNCTIONAL AND STRUCTURAL HOMOLOGY OF NATURAL AND RECOMBINANT HUMAN-LEUKOCYTE INTERFERON (IFN-ALPHA) WITH HUMAN ALPHA-ACTH AND BETA-ENDORPHIN [J].
EPSTEIN, LB ;
ROSE, ME ;
MCMANUS, NH ;
LI, CH .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1982, 104 (01) :341-346