CHLOROQUINE INDUCES EMPTY CAPSID FORMATION DURING POLIOVIRUS ECLIPSE

被引：29

作者：

KRONENBERGER, P ^{[1
]}

VRIJSEN, R ^{[1
]}

BOEYE, A ^{[1
]}

机构：

[1] VRIJE UNIV BRUSSELS,DEPT MICROBIOL & HYG,LAARBEEKLAAN 103,B-1090 BRUSSELS,BELGIUM

来源：

JOURNAL OF VIROLOGY | 1991年 / 65卷 / 12期

关键词：

D O I：

10.1128/JVI.65.12.7008-7011.1991

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The poliovirus capsid (160S) is modified during eclipse in HeLa cells, which results in at least three types of particles having sedimentation coefficients of 135, 110, and 80S. The lysosomotropic agent chloroquine redirected the production of eclipse products from 135 and 110S particles (containing RNA) to 80S particles (without RNA). The effect started at 5-mu-M and was fully developed with 20-mu-M chloroquine. Viral protein synthesis and virion production remained unaffected. The results show that chloroquine can redirect the processing of input virions without interfering with productive uncoating.

引用

页码：7008 / 7011

页数：4

共 13 条

[1] ECLIPSE PRODUCTS OF POLIOVIRUS AFTER COLD-SYNCHRONIZED INFECTION OF HELA-CELLS [J].

EVERAERT, L ;

VRIJSEN, R ;

BOEYE, A .

VIROLOGY, 1989, 171 (01) :76-82

[2] KINETICS OF POLIOVIRUS UNCOATING IN HELA-CELLS IN A NONACIDIC ENVIRONMENT [J].

GROMEIER, M ;

WETZ, K .

JOURNAL OF VIROLOGY, 1990, 64 (08) :3590-3597

[3] INHIBITION OF SEMLIKI FOREST VIRUS PENETRATION BY LYSOSOMOTROPIC WEAK BASES [J].

HELENIUS, A ;

MARSH, M ;

WHITE, J .

JOURNAL OF GENERAL VIROLOGY, 1982, 58 (JAN) :47-61

[4]

KOCH F, 1985, MOL BIOL POLIOVIRUS, P379

[5] DIFFERENTIATION OF ACID-PH-DEPENDENT AND ACID-PH-NONDEPENDENT ENTRY PATHWAYS FOR MOUSE HEPATITIS-VIRUS [J].

KOOI, C ;

CERVIN, M ;

ANDERSON, R .

VIROLOGY, 1991, 180 (01) :108-119

[6]

KRONENBERGER P, UNPUB

[7] MECHANISM OF ENTRY INTO THE CYTOSOL OF POLIOVIRUS TYPE-1 - REQUIREMENT FOR LOW PH [J].

MADSHUS, IH ;

OLSNES, S ;

SANDVIG, K .

JOURNAL OF CELL BIOLOGY, 1984, 98 (04) :1194-1200

[8] CHLOROQUINE ENHANCES REPLICATION OF SEMLIKI FOREST VIRUS AND ENCEPHALOMYOCARDITIS VIRUS IN MICE [J].

MAHESHWARI, RK ;

SRIKANTAN, V ;

BHARTIYA, D .

JOURNAL OF VIROLOGY, 1991, 65 (02) :992-995

[9] WEAK BASES AND IONOPHORES RAPIDLY AND REVERSIBLY RAISE THE PH OF ENDOCYTIC VESICLES IN CULTURED MOUSE FIBROBLASTS [J].

MAXFIELD, FR .

JOURNAL OF CELL BIOLOGY, 1982, 95 (02) :676-681

[10] CELLULAR RECEPTOR FOR POLIOVIRUS - MOLECULAR-CLONING, NUCLEOTIDE-SEQUENCE, AND EXPRESSION OF A NEW MEMBER OF THE IMMUNOGLOBULIN SUPERFAMILY [J].

MENDELSOHN, CL ;

WIMMER, E ;

RACANIELLO, VR .

CELL, 1989, 56 (05) :855-865

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