MICROBIAL-PRODUCTION OF D-MALATE FROM MALEATE

被引:23
作者
ASANO, Y
UEDA, M
YAMADA, H
机构
[1] MITSUBISHI KASEI CORP,RES CTR,MIDORI KU,YOKOHAMA 227,JAPAN
[2] KANSAI UNIV,FAC ENGN,SUITA,OSAKA 564,JAPAN
关键词
D O I
10.1128/AEM.59.4.1110-1113.1993
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
To prodUCe D-malate from maleate by a microbial reaction, we screened a number of maleate-utilizing microorganisms for enzyme activity by an intact cell system. The strain which showed the best productivity among the 440 strains tested was identified taxonomically as Arthrobacter sp. strain MCI2612. The optical purity of the malate produced by this strain was 100% D type. The culture and reaction conditions for the production were studied for this strain. Addition of amino acids such as L-proline, L-histidine, and L-arginine to the culture medium promoted the formation of reaction activity as well as cell growth. Under optimum conditions, 87 g of D-malate per liter was produced in 20 h. The yield was 72 mol%.
引用
收藏
页码:1110 / 1113
页数:4
相关论文
共 11 条