A PRELIMINARY TRIAL OF HUMAN ZYGOTE CULTURE IN HAM F-10 WITHOUT HYPOXANTHINE

被引：10

作者：

LOUTRADIS, DC ^{[1
]}

KIESSLING, AA ^{[1
]}

KALLIANIDIS, K ^{[1
]}

SISKOS, K ^{[1
]}

CREATSAS, G ^{[1
]}

MICHALAS, S ^{[1
]}

ARAVANTINOS, D ^{[1
]}

机构：

[1] UNIV ATHENS,ALEXANDRA MATERN HOSP,SCH MED,DEPT OBSTET & GYNECOL,ATHENS,GREECE

来源：

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS | 1993年 / 10卷 / 04期

关键词：

IN-VITRO FERTILIZATION (IVF); CULTURE MEDIUM; HYPOXANTHINE; EARLY EMBRYO DEVELOPMENT;

D O I：

10.1007/BF01204941

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Purpose: This study deals with the suitability of Ham's F-10 without hypoxanthine for early cleavage-stage embryo culture. A high percentage of mouse two-cell embryos developed into morula and blastocysts in Ham's F-10 formulated without hypoxanthine (75.3 and 71.6%, respectively); in contrast, in agreement with previous reports, only 15.4% developed beyond the two-cell stage in Ham's F-10 with hypoxanthine. To begin to evaluate the effect of hypoxanthine on human embryos, a total of 318 human oocytes was fertilized and cultured in Ham's F-10 minus hypoxanthine. Results: The fertilization, cleavage, and pregnancy percentages in two ovulation induction protocols [human menopausal gonadotropin (hMG)/human chorionic gonadotropin (hCG) and gonadotropin releasing hormone/hMG/hCG] were 82, 88, and 20 and 83, 91, and 30%, respectively. Conclusion: These results suggest that, in agreement with mouse embryo development, hypoxanthine does not appear to be necessary for human embryo cleavage and its omission from Ham's F-10 may enhance the conditions for culture of early-cleaving human embryos. These observations may lead to a better understanding of critical cell processes during early human embryonic development.

引用

页码：271 / 275

页数：5

共 20 条

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