NERVE GROWTH-FACTOR (NGF), BRAIN-DERIVED NEUROTROPHIC FACTOR (BDNF) AND LOW-AFFINITY NERVE GROWTH-FACTOR RECEPTOR (LNGFR) MESSENGER-RNA LEVELS IN CULTURED RAT SCHWANN-CELLS - DIFFERENTIAL TIME-DEPENDENT AND DOSE-DEPENDENT REGULATION BY CAMP

被引：64

作者：

YAMAMOTO, M

SOBUE, G

LI, M

ARAKAWA, Y

MITSUMA, T

KIMATA, K

机构：

[1] AICHI MED UNIV,DEPT INTERNAL MED 4,DIV NEUROL,NAGAKUTE,AICHI 48011,JAPAN

[2] CHUBU NATL HOSP,DEPT CLIN RES,AICHI,JAPAN

[3] EISAI PHARMACEUT CO,TSUKUBA CENT RES LAB,TSUKUBA,JAPAN

[4] AICHI MED UNIV,INST MOLEC SCI MED,NAGAKUTE,AICHI 48011,JAPAN

来源：

NEUROSCIENCE LETTERS | 1993年 / 152卷 / 1-2期

关键词：

NERVE GROWTH FACTOR; BRAIN-DERIVED NEUROTROPHIC FACTOR; LOW-AFFINITY NERVE GROWTH FACTOR RECEPTOR; CAMP; GENE EXPRESSION; SCHWANN CELL;

D O I：

10.1016/0304-3940(93)90477-3

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Steady-state mRNA levels for NGF, BDNF, and NT-3, and their receptors, LNGFR, trk, trkB, and trkC, were examined in cultured rat Schwann cells. NGF, BDNF, and LNGFR mRNAs were expressed in control Schwann cells, but NT-3, trk, trkB and trkC mRNAs were not. As previously reported, when Schwann cells were treated with forskolin or 8-bromo-cAMP, NGF mRNA expression was enhanced, while BDNF and LNGFR mRNA levels were suppressed. The optimal concentration of forskolin for NGF mRNA enhancement and BDNF mRNA suppression was similar to that for LNGFR mRNA suppression, which coincided with the optimal concentration for Schwann cells to differentiate. However, the lag-time for forskolin-induces down-regulation of LNGFR mRNA was far greater than that for NGF mRNA increase and BDNF mRNA suppression.

引用

页码：37 / 40

页数：4

共 22 条

[1] DETECTION OF BRAIN-DERIVED NEUROTROPHIC FACTOR-LIKE ACTIVITY IN FIBROBLASTS AND SCHWANN-CELLS - INHIBITION BY ANTIBODIES TO NGF [J].