CLINICAL-EVALUATION OF RAPID SERODIAGNOSIS OF PULMONARY TUBERCULOSIS BY ELISA WITH CORD FACTOR (TREHALOSE-6,6'-DIMYCOLATE) AS ANTIGEN PURIFIED FROM MYCOBACTERIUM-TUBERCULOSIS

被引：47

作者：

MAEKURA, R ^{[1
]}

NAKAGAWA, M ^{[1
]}

NAKAMURA, Y ^{[1
]}

HIRAGA, T ^{[1
]}

YAMAMURA, Y ^{[1
]}

ITO, M ^{[1
]}

UEDA, E ^{[1
]}

YANO, S ^{[1
]}

HE, H ^{[1
]}

OKA, S ^{[1
]}

KASHIMA, K ^{[1
]}

YANO, I ^{[1
]}

机构：

[1] OSAKA CITY UNIV,SCH MED,DEPT BACTERIOL,OSAKA 545,JAPAN

来源：

AMERICAN REVIEW OF RESPIRATORY DISEASE | 1993年 / 148卷 / 04期

关键词：

D O I：

10.1164/ajrccm/148.4_Pt_1.997

中图分类号：

R56 [呼吸系及胸部疾病];

学科分类号：

摘要：

Immunoglobulin G (IgG) antibodies against purified cord factor (trehalose-6,6'-dimycolate) prepared from Mycobacterium tuberculosis H37Rv were determined by enzyme-linked immunosorbent assay (ELISA), and its diagnostic usefulness was evaluated. Serum specimens from 65 patients with active pulmonary tuberculosis, 58 patients with inactive pulmonary tuberculosis, 36 patients with diseases other than tuberculosis, and 66 healthy adults were examined. Patients with active pulmonary tuberculosis showed significantly higher titers of IgG antibodies against cord factor than did other groups (p < 0.001). The antibody titer greater than 0.29 in absorption difference (492 to 630 nm) of 160-times diluted serum was set as positive in ELISA. For patients with active and untreated pulmonary tuberculosis, the ELISA had a sensitivity of 81% and a specificity of 96%. From these results, it was concluded that the detection of IgG antibodies against cord factor is useful for serodiagnosis of active pulmonary tuberculosis. It was also indicated that the anticord factor antibody titers decline to the normal level as a result of antituberculosis chemotherapy.

引用

页码：997 / 1001

页数：5

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