TFIID SEQUENCE RECOGNITION OF THE INITIATOR AND SEQUENCES FARTHER DOWNSTREAM IN DROSOPHILA CLASS-II GENES

Immunopurified TEIID produces a large DNase I footprint over the hsp70, hsp26, and histone H3 promoters of Drosophila. These footprints span from the TATA element to a position similar to 35 nucleotides downstream from the transcription start site. Using a ''missing nucleoside'' analysis, four regions within the three promoters have been found to be important for TFIID binding: the TATA element, the initiator, and two regions located similar to 18 and 28 nucleotides downstream of the transcription start site. On the basis of the missing nucleoside data, the initiator appears to contribute as much to the affinity as the TATA element. However, there is weak conservation of the sequence in this region. To determine whether a preferred binding sequence exists in the vicinity of the initiator, the nucleotide composition of this region within the hsg70 promoter was randomized and then subjected to selection by TFIID. After five rounds of selection, the preferred sequence motif-(G)/A/(T) (C)/(T) AT/(G) T G-emerged. This motif is a close match to consensus sequences that have been derived by comparing the initiator region of numerous insect promoters. Selection of this sequence demonstrates that sequence-specific interactions downstream of the TATA element contribute to the interaction of TFIID on a wide spectrum of promoters.