ALTERNATE SPLICING OF HUMAN THROMBOXANE SYNTHASE MESSENGER-RNA

被引：20

作者：

WANG, LH ^{[1
]}

TAZAWA, R ^{[1
]}

LANG, AQ ^{[1
]}

WU, KK ^{[1
]}

机构：

[1] UNIV TEXAS, HLTH SCI CTR, DEPT INTERNAL MED, VASC BIOL RES CTR, HOUSTON, TX 77030 USA

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1994年 / 315卷 / 02期

关键词：

D O I：

10.1006/abbi.1994.1500

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two species of human thromboxane synthase (TXS) cDNA, called TXS-I and -II, were previously isolated (K. Ohashi, K.-H. Ruan, R. J. Kulmacz, K. K. Wu, and L.-H. Wang, 1992, J. Biol. Chem. 267, 789-793). TXS-II differs from TXS-I by a 163-bp deletion near the 3'-end of the coding region. Both types of TXS mRNA have now been demonstrated to be present in various blood and lung cultured cells. Analysis of the exon-intron boundaries of TXS genomic DNA revealed that the two mRNAs are generated via alternate splicing: TXS-II is produced by skipping an entire 163-bp exon which encodes the polypeptide segment containing the heme-binding cysteine conserved among other P450s. The mechanism by which alternate splicing occurs is probably due to the presence of a more powerful potential as the 3' acceptor site in the intron following the 163-bp exon. When expressed in baculovirus system, recombinant TXS-I catalyzed the formation of thromboxane Az and 12-hydroxyheptadecatrienoic acid (HHT), whereas recombinant TXS-II did not synthesize thromboxane Aa or HHT. Alternate splicing of TXS RNA transcript thus may provide a mechanism for limiting cellular biosynthesis of thromboxane Az. (C) 1994 Academic Press, Inc.

引用

页码：273 / 278

页数：6

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