PLAQUE-ASSAY FOR THE SIMIAN ROTAVIRUS-SAII

被引：120

作者：

SMITH, EM ^{[1
]}

ESTES, MK ^{[1
]}

GRAHAM, DY ^{[1
]}

GERBA, CP ^{[1
]}

机构：

[1] BAYLOR UNIV,COLL MED,DEPT VIROL & EPIDEMIOL,HOUSTON,TX 77030

来源：

JOURNAL OF GENERAL VIROLOGY | 1979年 / 43卷 / JUN期

关键词：

D O I：

10.1099/0022-1317-43-3-513

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A sensitive, quantitative and reproducible plaque assay for the measurement of the simian rotavirus SA(II) is described. Plaque formation required the presence of the facilitators pancreatin or trypsin and diethylaminoethyl-dextran in the agar overlay. SA(II) produced plaques in three continuous primate cell lines: MA-104, CV-1 and LLC-MK2. MA-104 cells were the most sensitive.

引用

页码：513 / 519

页数：7

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