IMMUNOCHEMICAL STUDIES OF PHOSPHOLIPIDS - PRODUCTION OF ANTIBODIES TO TRIPHOSPHOINOSITIDE

被引：9

作者：

GREENBERG, AJ

TREVOR, AJ

JOHNSON, DA

LOH, HH

机构：

[1] Department of Pharmacology, School of Medicine, University of California, San Francisco

来源：

MOLECULAR IMMUNOLOGY | 1979年 / 16卷 / 03期

关键词：

D O I：

10.1016/0161-5890(79)90145-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Antibodies to triphosphoinositide (TPI, I-phosphatidylinositol-3,4-bisphosphate) were raised in rabbits immunized with a hapten suspension of TPI, lecithin and cholesterol (1:4:30 by wt) in 0.1% methylated BSA. The hapten suspension was administered i.v. every two days for three weeks with each rabbit receiving a total of 3 mg TPI. Immunodiffusion with the partially purified immunoglobulin fraction and the comparison of complement-isofixation curves demonstrate complete cross-reactivity between TPI and diphosphoinositide (DPI) and very little specificity towards phosphatidylinositol (PI) and cardiolipin. © 1979.

引用

页码：193 / 196

页数：4

共 24 条

[1] TOPOLOGICAL ASYMMETRY OF PHOSPHOLIPIDS IN MEMBRANES [J].