SENSITIVE PHOTOMETRIC ASSAY FOR ALCOHOL-DEHYDROGENASE ACTIVITY IN BLOOD-SERUM

被引：87

作者：

SKURSKY, L

KOVAR, J

STACHOVA, M

机构：

[1] Department of Biochemistry, Faculty of Science, Purkyně University, 611 37 Brno

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 99卷 / 01期

关键词：

D O I：

10.1016/0003-2697(79)90044-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Minute activities of alcohol dehydrogenase in blood serum can be determined from the fading of the yellow color of p-nitrosodimethylaniline in a recycling reaction. This aldehyde-like substrate is reduced enzymatically to a colorless compound in the presence of NADH which is generated by alcohol dehydrogenase from NAD and n-butanol. Using this method alcohol dehydrogenase activities can also be measured in other biological materials. © 1979.

引用

页码：65 / 71

页数：7

共 14 条

[1]

Branden C-I., 1975, ENZYMES, V11, P103, DOI 10.1016/S1874-6047(08)60211-5

[2]

CARTER EA, 1977, P SOC EXP BIOL MED, V155, P290

[3] THE ASSAY AND SPECIFIC ACTIVITY OF CRYSTALLINE ALCOHOL DEHYDROGENASE OF HORSE LIVER [J].