CAMP LEVELS AND INSITU MEASUREMENT OF CAMP RELATED ENZYMES DURING YEAST-TO-HYPHAE TRANSITION IN CANDIDA-ALBICANS

被引：20

作者：

EGIDY, G

PAVETO, C

PASSERON, S

GALVAGNO, MA

机构：

[1] UNIV BUENOS AIRES,FAC CIENCIAS EXACTAS & NAT,DEPT QUIM BIOL,PABELLON 2,RA-1428 BUENOS AIRES,ARGENTINA

[2] UNIV BUENOS AIRES,FAC CIENCIAS EXACTAS & NAT,DEPT CIENCIAS BIOL,RA-1428 BUENOS AIRES,ARGENTINA

来源：

CELL BIOLOGY INTERNATIONAL REPORTS | 1990年 / 14卷 / 01期

关键词：

D O I：

10.1016/0309-1651(90)90071-6

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Intracellular levels of cAMP and specific activities of adenylate cyclase, cAMP phosphodiesterase and cAMP-dependent protein kinase were measured during filamentation in the dimorphic fungus Candida albicans. Enzymatic assays were performed in permeabilized cells under conditions prevented endogenous proteolysis. The variations observed in cAMP levels were mainly accounted for by variations in the specific activities of adenylate cyclase and cAMP phosphodiesterase at different stages during germ tube formation. cAMP-dependent protein kinase, measured with kemptide as exogenous substrate, was developmental regulated. Some properties of the enzymatic activities from cell-free extracts are described. © 1990.

引用

页码：59 / 68

页数：10

共 20 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2] KINETIC-PROPERTIES, SOLUBILIZATION, AND MOLECULAR CHARACTERIZATION OF MUCOR-ROUXII ADENYLATE-CYCLASE [J].