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FLOW CYTOMETRY - HIGH-RESOLUTION INSTRUMENT FOR EVERYONE

被引：133
作者：
STEEN, HB
LINDMO, T
机构：
[1] Department of Biophysics, Norsk Hydro's Institute for Cancer Research, Montebello
来源：
SCIENCE | 1979年 / 204卷 / 4391期
关键词：
D O I：
10.1126/science.441727
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A new flow configuration for flow cytometry has been devised in which a flat, laminar stream of water, containing the stained cells in a narrow central sector, is formed on a microscope cover slip by a pressurized jet of water directed onto the glass at low angle. The stream of cells is viewed by means of a fluorescence microscope with incident illumination and a pulse photometer. Coupled to a multichannel pulse height analyzer, the instrument constitutes a stable and easy-to-operate flow cytometer with a resolution equal to or better than a coefficient of variance of 1.4 percent in measurements of cellular DNA. Copyright © 1979 AAAS.
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页码：403 / 404
页数：2
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