DETECTION OF STRAINS OF POTATO VIRUS-S BY NUCLEIC-ACID SPOT HYBRIDIZATION (NASH)

被引：11

作者：

FOSTER, GD

MILLS, PR

机构：

[1] QUEENS UNIV BELFAST,DEPT MYCOL & PLANT PATHOL,BELFAST BT9 5PX,ANTRIM,NORTH IRELAND

[2] DEPT AGR NO IRELAND,DIV PLANT PATHOL RES,BELFAST BT9 5PX,NORTH IRELAND

来源：

POTATO RESEARCH | 1990年 / 33卷 / 04期

关键词：

PVS; ELISA; CDNA; CLONING; SOLANUM-TUBEROSUM L;

D O I：

10.1007/BF02358027

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Two complementary DNA (cDNA) clones (0.9 kb and 1.6 kb) reacting to the ordinary strain of potato virus S (PVS) were compared with single-stranded randomly primed cDNA (prepared to total genomic RNA) as probes for various strains of PVS, using the technique of nucleic acid spot hybridization (NASH). The cDNA clones detected 11 PVS isolates well, including both Andean and ordinary strains, while one of each strain was detected weakly. As little as 5 femtograms of PVS RNA could be detected. A low cross hybridization was observed with cDNA probes to PVS with potato virus M and carnation latent virus both members of the carlavirus group. No cross homology was detected with seven other potato viruses. NASH was as sensitive and specific as enzyme-linked immunosorbent assay (ELISA), with no pretreatments of crude sap being required before spotting on nitrocellulose filters. Cloned cDNA probes would be suitable for mass screening of PVS.

引用

页码：487 / 495

页数：9

共 17 条

[1] THE USE OF F(AB')2-BASED ELISA TO DETECT SEROLOGICAL RELATIONSHIPS AMONG CARLAVIRUSES [J].