PLANT TRANSFORMATION - A SIMPLE PARTICLE BOMBARDMENT DEVICE BASED ON FLOWING HELIUM

被引：70

作者：

TAKEUCHI, Y ^{[1
]}

DOTSON, M ^{[1
]}

KEEN, NT ^{[1
]}

机构：

[1] UNIV CALIF RIVERSIDE,DEPT PLANT PATHOL,RIVERSIDE,CA 92521

来源：

PLANT MOLECULAR BIOLOGY | 1992年 / 18卷 / 04期

关键词：

TRANSFORMATION; PARTICLE GUN; SOYBEAN; GUS GENE;

D O I：

10.1007/BF00020031

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We observed that flowing helium at moderate pressures accelerated DNA-coated microprojectiles to velocities suitable for penetration of cells in intact plant tissues. The flowing helium principle permitted the construction of a simple and inexpensive transformation device that was easier to use than those previously described. This device provided efficient transformation of cells in soybean seedlings and other plants.

引用

页码：835 / 839

页数：5

共 10 条

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